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myc ciap1  (Addgene inc)


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    Structured Review

    Addgene inc myc ciap1
    Myc Ciap1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+ciap1/pmc11268115-293-9-13?v=Addgene+inc
    Average 88 stars, based on 7 article reviews
    myc ciap1 - by Bioz Stars, 2026-07
    88/100 stars

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    (A) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type ubiquitin (Ub), K48 Ub, K63 Ub, FLAG-tagged IRF4, V5-tagged RICK, and TRAF6; whole cell lysates were prepared 48 hours after the transfection and subjected to immunoprecipitation (IP) with the indicated Ab followed by immunoblotted (IB) with the indicated Ab. (B) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing FLAG-tagged IRF4, Myc-DDK-tagged <t>cIAP1,</t> Myc-DDK-tagged cIAP2, V5-tagged RICK, and HA-tagged K48 or K63 Ub; whole cell lysates were subjected to IP with the indicated Ab followed by IB with the indicated Ab. (C) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type Ub, V5-tagged RICK, TRAF6, FLAG-tagged IRF4, FLAG-tagged mutated IRF4 (Mut IRF4) in which serine residues at 447 and 448 are replaced with alanine; whole cell lysates were prepared 48 hours after the transfection and subjected to IP with the indicated Ab followed by IB with the indicated Ab. (D) 293 cells stably expressing NOD2 (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type Ub, FLAG-tagged IRF4, and V5-tagged RICK. 48 hours after the transfection, cells were stimulated with MDP (10 μg/ml) for 30 min; whole cell lysates were subjected to IP with the indicated Ab followed by IB with the indicated Ab. Results shown are representative of two experiments.
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    Image Search Results


    (A) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type ubiquitin (Ub), K48 Ub, K63 Ub, FLAG-tagged IRF4, V5-tagged RICK, and TRAF6; whole cell lysates were prepared 48 hours after the transfection and subjected to immunoprecipitation (IP) with the indicated Ab followed by immunoblotted (IB) with the indicated Ab. (B) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing FLAG-tagged IRF4, Myc-DDK-tagged cIAP1, Myc-DDK-tagged cIAP2, V5-tagged RICK, and HA-tagged K48 or K63 Ub; whole cell lysates were subjected to IP with the indicated Ab followed by IB with the indicated Ab. (C) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type Ub, V5-tagged RICK, TRAF6, FLAG-tagged IRF4, FLAG-tagged mutated IRF4 (Mut IRF4) in which serine residues at 447 and 448 are replaced with alanine; whole cell lysates were prepared 48 hours after the transfection and subjected to IP with the indicated Ab followed by IB with the indicated Ab. (D) 293 cells stably expressing NOD2 (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type Ub, FLAG-tagged IRF4, and V5-tagged RICK. 48 hours after the transfection, cells were stimulated with MDP (10 μg/ml) for 30 min; whole cell lysates were subjected to IP with the indicated Ab followed by IB with the indicated Ab. Results shown are representative of two experiments.

    Journal: Mucosal immunology

    Article Title: NOD2 Down-Regulates Colonic Inflammation by IRF4-Mediated Inhibition of K63-Linked Polyubiquitination of RICK and TRAF6

    doi: 10.1038/mi.2014.19

    Figure Lengend Snippet: (A) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type ubiquitin (Ub), K48 Ub, K63 Ub, FLAG-tagged IRF4, V5-tagged RICK, and TRAF6; whole cell lysates were prepared 48 hours after the transfection and subjected to immunoprecipitation (IP) with the indicated Ab followed by immunoblotted (IB) with the indicated Ab. (B) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing FLAG-tagged IRF4, Myc-DDK-tagged cIAP1, Myc-DDK-tagged cIAP2, V5-tagged RICK, and HA-tagged K48 or K63 Ub; whole cell lysates were subjected to IP with the indicated Ab followed by IB with the indicated Ab. (C) 293 cells (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type Ub, V5-tagged RICK, TRAF6, FLAG-tagged IRF4, FLAG-tagged mutated IRF4 (Mut IRF4) in which serine residues at 447 and 448 are replaced with alanine; whole cell lysates were prepared 48 hours after the transfection and subjected to IP with the indicated Ab followed by IB with the indicated Ab. (D) 293 cells stably expressing NOD2 (1×10 6 /6 well plate) were transfected with vectors (1 μg) expressing HA-tagged wild type Ub, FLAG-tagged IRF4, and V5-tagged RICK. 48 hours after the transfection, cells were stimulated with MDP (10 μg/ml) for 30 min; whole cell lysates were subjected to IP with the indicated Ab followed by IB with the indicated Ab. Results shown are representative of two experiments.

    Article Snippet: HEK293 cells (ATCC) (1×10 6 /cells) were transfected with 1 μg of FLAG-tagged human IRF4 vector , HA-tagged human MyD88, TRAF6 (InvivoGen), V5-tagged RICK , Myc-DDK-tagged cIAP1, Myc-DDK-tagged cIAP2 (Origene), HA-tagged wild type ubiquitin (Ub), K63 Ub, K48 Ub (kindly provided by Dr. J. Chen), and His-tagged Ub (kindly provided by Dr. H. Kondoh) by Lipofectamine 2000 (Invitrogen) or by Fugene 6 (Promega).

    Techniques: Transfection, Expressing, Immunoprecipitation, Stable Transfection